Measurement of Insulin Sensitivity in Children

RESEARCH DESIGN AND METHODS — The subjects were Minneapolis fifthto eighth-grade students. Euglycemic-hyperinsulinemic clamps were performed on 323 adolescents at mean age 13 and were repeated on 300 of these subjects at mean age 15. Insulin sensitivity was determined by glucose uptake (milligrams per kilogram per minute) adjusted for lean body mass (MLBM) and steady-state insulin (MLBM/ln SSI). Comparisons were made for the whole cohort and by body size (BMI 85th percentile vs. BMI 85th percentile). Receiver operating characteristic (ROC) curves were used to test whether specific fasting insulin cut points separated true-positive from false-positive approximations of insulin resistance.